TCO-PEG-Val-Ala-PAB-PNP

Trans-cyclooctene-functionalized discrete poly(ethylene glycol)-valyl-alanine-p-aminobenzyl p-nitrophenyl carbonate (TCO-PEG-Val-Ala-PAB-PNP) is an ADC linker intermediate containing a defined-length PEG spacer. It contains a trans-cyclooctene (TCO) reactive terminus and a valine–alanine dipeptide. Discrete chain lengths with different degrees of polymerization are available. Trans-cyclooctene can pair with tetrazines in inverse-electron-demand Diels–Alder reactions for assembly of bioorthogonal modules. The valine–alanine dipeptide provides a sequence variable for studies of protease-cleavable linkers. The PAB spacer links peptide cleavage to the subsequent self-immolation process. The PNP-activated terminus can be further coupled to suitable amine-containing payloads.

Customization available.